Optical imaging is the imaging of objects with visible light. It is a tool often used for diagnostic purposes, such as in biomedical and material sciences. Digital holography is an optical imaging technique that captures and images the amplitude as well as the phase (the complex amplitude) of the lightwave. An advantage is that the complex amplitude can be calculated in different propagation planes. The goal of this thesis is to use digital holography to image depth of a reflecting surface aswell asmake 3D images of biological samples, and to contribute to the theoretical understanding in this regard.

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